Check out the Special Issue in ABB:
"Flavoproteins: Beyond the classical paradigms"
Google Scholar-Pubmed
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1703060.pdf |
2022
88. Dye, KJ, Vogelaar, NJ, O'Hara, M, Sobrado, P, Santos W, Carlier PR, Yang Z. (2022) Discovery of Two Inhibitors of the Type IV Pilus Assembly ATPase PilB as Potential Antivirulence Compounds. Microbiol Spectr. Nov 15:e0387722. doi: 10.1128/spectrum.03877-22.
87. Lyons, NS, Bogner, AN, Tanner, JJ, Sobrado, P. (2022) Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry. 2022 Nov 15;61(22):2607-2620. doi: 10.1021/acs.biochem.2c00493.
proof_8_.pdf |
2021
86. Sobrado, P. (2021) Role of reduced flavin in dehalogenation reactions. Arch. Biochem. Biophys. 699:108765. doi: 10.1016/j.abb.2020.108696
2hah_review_finala.pdf |
85. Dye, K.J., Vogelaar, N.J., Sobrado, P., Yang, Z. (2021) High-throughput screen for inhibitors of the Type IV Pilus Assembly ATPase PiB. 6: e00129-2. doi: 10.1128/mSphere.00129-21
84. Valentino, H., Korasick, A.D., Bohac, T.J., Shapiro, J.A., Wencewicz, A.T., Tanner, J.J., and Sobrado, P. (2021) ACS Omega 6(28):18537-18547. doi: 10.1021/acsomega.1c03047
proof_6_.pdf |
83. Valentino, H., and Sobrado, P. (2021) Characterization of nitro-forming enzyme involved in fosfazinomycin biosynthesis. Biochemistry. 60:2851-2864. doi: 10.1021/acs.biochem.1c00512
proof_7_.pdf |
2020
82. Reis, R., Li, H., Johnson, M., Sobrado, P. Frontier in flavin-dependent monooxygenases. Arch. Biochem. Biophys. In press
sidareview2021.pdf |
81. Li, H., Forson, B., Eckshtain-Levi, M., Valentino, H., Martin Del Campo, J., Tanner, J.J., Sobrado, P. Biochemical Characterization of the Two-Component Flavin-Dependent Monooxygenase involved in Valanimycin Biosynthesis. Biochemistry, In press, 2020. doi: 10.1021/acs.biochem.0c00679
acs.biochem.0c00679.pdf |
80. Campbell, A.C., Robinson, R., Mena-Aguilar, D., Sobrado P., Tanner, J.J. (2020) Structural determinants of flavin dynamics in a class B flavin-dependent monooxygenase. Biochemistry. 59::4609-4616.
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79. Sobrado P. (2020) Role of reduced flavin in dehalogenation reactions (2020). Arch. Biochem. Biophys.
2hah_review_final.pdf |
78. Campbell AC, Stiers, K.M., Martin Del Campo, J.S., Mehra-Chaudhary, R., Sobrado P. Tanner JJ, (2020) Trapping conformatioinal states of a flavin-dependent N-monooxygenase in crystallo reveals protein and flavin dynamics. J. Biol. Chem. 295:13239-13249
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77. Valentino H, Campbell AC, Schuermann JP, Sultana N, Nam HG, LeBlanc S, Tanner JJ, Sobrado P. P Structure and Function of a Flavin-Dependent S-monooxygenase From Garlic (Allium Sativum). J. Biol. Chem. In press. DOI: 10.1074/jbc.RA120.014484
j._biol._chem.-2020-valentino-jbc.ra120.014484.pdf |
76. Hofmann M, Martin Del Campo JS, Sobrado P, Tischler D. Biosynthesis of Desferrioxamine Siderophores Initiated by Decarboxylases: A Functional Investigation of Two lysine/ornithine-decarboxylases From Gordonia Rubripertincta CWB2 and Pimelobacter Simplex 3E
Arch Biochem Biophys. 2020 May 30;689:108429. DOI: 10.1016/j.abb.2020.108429
Arch Biochem Biophys. 2020 May 30;689:108429. DOI: 10.1016/j.abb.2020.108429
1-s2.0-s0003986120304380-main.pdf |
2019
75. Valentino H, Sobrado P. Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber. (2019). Methods Enzymol. 620:51-88. doi: 10.1016/bs.mie.2019.03.006.
pagination_mie_1274.pdf |
74. Cossio-P Eacute Rez R, Pierdominici-Sottile G, Sobrado P, Palma J. (2019). Molecular dynamics simulations of substrate release from Trypanosoma cruzi UDP_galatopyranose mutase. J. Chem. Inf. Model. 59(2):809-817. doi: 10.1021/acs.jcim.8b00675
acs.jcim.8b00675.pdf |
73. Robinson, R., Klancher, C., Rodriguez, P., and Sobrado, P. (2018) Flavin oxidation in flavin dependent N-monooxygenase. Protein Science (2019). 28: 90-99
robinson_et_al-2018-protein_science.pdf |
2018
72. Mehnert, M., Heine, T., Sobrado, P., Tischler, D., (2019) Identification of a gene cluster involved in desferrioxamine biosynthesis in Gordonia rubripertincta and Pimelobacter simplex. Exploring Microorganism: Recent Advancements in Applied Microbiology (Mendez-Vilas, A. Ed) BrownWalker
71. Dai, Y., and Sobrado, P. (2018) Evidence for Formation of a Radical-Mediated Flavin-N5 Covalent Intermediate (2018). ChemBioChem. 19:1609-1612
dai_et_al-2018-chembiochem.pdf |
70. Pierdominicci-Sotteli, G., Cossio-Perez, R., Da Fonseca, L, Kizjakina, K., Tanner, J.J., and Sobrado, P. (2018) Steric Control of the Rate Limiting-Step of UDP-Galactopyranose Mutase. Biochemistry. 57:3713-3721.
acs.biochem.8b00323.pdf |
69. Gadda, G., and Sobrado, P. (2018) Kinetic Solvent Viscosity Effects as Probes to Study the Mechanisms Enzyme Action. Biochemistry. Biochemistry. 57:3445-34453.
acs.biochem.8b00232.pdf |
68. Li-Kai, L., Dai, Y., Abdelwahad, H., and Sobrado, P., Tanner, J.J. (2018) Structural evidence for rifampicin monooxygenase inactivating rifampicin by cleaving its ansa-bridge. Biochemistry. 57:2065-68.
acs.biochem.8b00190.pdf |
67. Dai, Y., Kizjakina, K., Campbell, A.C., Korasick. D.A., Tanner, J.J, and Sobrado, P. (2018) Flavin-N5 Covalent Intermediate in the Non-redox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme. ChemBioChem. 19:53-57.
2hahreprint.pdf |
2017
66. Sobrado, P., and Gadda, G. (2017) Introduction to flavoproteins: Beyond the classical paradigms. Arch. Biochem. Biophys. 632:1-3
1-s2.0-s0003986117306094-main.pdf |
65. Bufkin, K., and Sobrado, P. (2017) Characterization of the Ornithine Hydroxylation Step in Albachelin Biosynthesis. Molecules 22: 1652.
final.pdf |
64. Martin Del Campo, J.S., M. Eckshtain-Levi, and Sobrado, P. (2017) Identification of eukaryotics UDP-galactopyranose mutase inhibitors using the ThermoFAD assay. Biochem. Biopys. Res. Cummun. 493:58-63
1-s2.0-s0006291x17318454-main-1.pdf |
63. Martín del Campo,J.S., Eckshtain-Levi, M., Vogelaar, N., Sobrado, P., (2017) Identification of Aspergillus fumigatus UDP-galactopyranose mutase inhibitors. Sci. Rep. 7:10836
ugm_hts_.pdf |
62. Webb, B., Compton, K., Martin Del Campo, J.S., Taylor, D., Sobrado, P., Scharf, B.E. (2017) Sinorhizobium meliloti chemotaxis to multiple amino acids is mediated by chemoreceptor McpU. Mol. Plat. Microbe. Interact. 10:770-777.
mpmi-04-17-0096-r.pdf |
61. Sobrado, P., and Tanner, J.J. (2017) Multiple functionalities of reduced flavin in the non-redox reaction catalyzed by UDP-galactopyranose mutase. Arch. Biochem. Biophys. 632:59-65.
sobrado_tanner.pdf |
2016
60. Abdelwahab H, Martin Del Campo JS, Dai Y, Adly C, El-Sohaimy S, Sobrado P. Mechanism of Rifampicin Inactivation in Nocardia farcinica.PLoS One. 2016 Oct 5;11(10):e0162578. doi: 10.1371/journal.pone.0162578.
journal.pone.0162578.pdf |
59. Liu LK, Abdelwahab H, Martin Del Campo JS, Mehra-Chaudhary R, Sobrado P., Tanner JJ. The Structure of the Antibiotic Deactivating, N-hydroxylating Rifampicin Monooxygenase. J Biol Chem. 2016 Oct 7;291(41):21553-21562.
rifmostructurejbc2016.pdf |
58. Martín Del Campo JS, Vogelaar N, Tolani K, Kizjakina K, Harich K, Sobrado P. (2016) Inhibition of the Flavin-Dependent Monooxygenase Siderophore A (SidA) Blocks Siderophore Biosynthesis and Aspergillus fumigatus Growth. ACS Chem Biol. 2016 Sep 15 Abstract
sida_acs_cb.pdf |
57. Abdelwahab H, Robinson R, Rodriguez P, Adly C, El-Sohaimy S, Sobrado P. (2016) Identification of structural determinants of NAD(P)H selectivity and lysine binding in lysine N(6)-monooxygenase. Arch Biochem Biophys. 2016 Sep 15;606:180-8. Abstract
heba_et_al.pdf |
56. Mehra-Chaudhary R, Dai Y, Sobrado P, Tanner JJ. (2016) In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction. Biochemistry. 55:833-6. Abstract
h63agalp.pdf |
2015
55. Robinson R, Qureshi IA, Klancher CA, Rodriguez PJ, Tanner JJ, Sobrado P. (2015) Contribution to catalysis of ornithine binding residues in ornithine N5-monooxygenase. Arch Biochem Biophys. 585:25-31.
orn_final.pdf |
54. Bai, Y., McCoy,J., Levin,E., Sobrado, P., Rajashankar, K., Fox, B.G., and Zhang, M. (2015) X-ray Structure of Mammalian Stearoyl-CoA desaturase. Nature. 524:252-6 (doi: 10.1038/nature14549).
nature14549.pdf |
53. Sohrabi, R., Huh, JH, Badieyan, S., Harinantenaina, L., Kingston DGI, Kliebensten, D., Sobrado, P., Tholl, D.,(2015) In Planta Variation of Volatile Biosynthesis: An alternative Biosynthetic Route to the Formation of the Pathogen-Induced Volatile Homoterpene DMNT via Triterpene Degradation in Arabidopsis Roots, Plant Cell. 27(3):874-90. Abstract
plant_cell-2015.pdf |
52. Binda, C., Robinson, R., Keul, N., Rodriguez, P., Robinson, H.H., Mattevi, A., Sobrado, P. (2015) An unprecedented NADPH-domain conformation in Lysine Monooxygenase NbtG provides insights into uncoupling of oxygen consumption from substrate hydroxylation. J. Biol. Chem.290. 12676-88. Abstract
jbcbinda2015.pdf |
51. Guo S, Liang Y, Murphy SF, Huang A, Shen H, Kelly DF, Sobrado P, Sheng Z. (2015) A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications. Autophagy. 1(3):560-72. Abstract
final.pdf |
50. Badieyan, S, Bach, R., Sobrado, P. (2015) Mechanism of N-Hydroxylation Catalyzed by Flavin-dependent Monooxygenases . J. Org. Chem. 80, :2139-47. Abstract
jo502651v-2.pdf |
2014
49. Da Fonseca, I., Qureshi, I.A., Mehra-Chaudharay, R., Kizjakina, K., Tanner, J.J., Sobrado, P. (2014) Contributions of Unique Active Site Residues of Eukaryotic UDP-Galactopyranose Mutases to Substrate Recognition and Active Site Dynamics. Biochemistry. 53, 7794-7804.
ugm_mutants_final.pdf |
48. Robinson, R., Rodriguez, P., Sobrado, P. (2014) Mechanistics studies on the flavin-dependent N6-lysine monooxygenase MbsG reveal an unusual control for catalysis. Arch. Biochem. Biophys. 550-551, 58-66.
1-s2.0-s0003986114001362-main.pdf |
47. Robinson, R., Franceschini, S., Fedkenheuer, M., Rodriguez, P., Ellerbrock, P., Romero, E., Echandi, M.P., Martin del Campo, J., Sobrado, P. (2014) Arg279 is the key regulator of coenzyme selectivity in the flavin-dependent ornithine monooxygenase SidA. Biochim. Biophys. Acta. 1844. 778-784.
sida279mutant.pdf |
46. Tanner, J., Boechi, L., McCammon, J.A., Sobrado, P. (2014) Structure, Mechanism, and Dynamics of UDP-Galactopyranose Mutase. Arch. Biochem. Biophys. 544. 128-141. Review. Selected for the Cover
ugmreview2014.pdf |
2013
45. Sobrado, P. (2013) Flavin-Dependent N-Hydroxylating monooxygenases in Bacterial and Fungi Siderophore Biosynthesis. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 265-276.Raleigh, NC: Lulu.
sobrado_p.pdf |
44. Oppenheimer, M., Valenciano, A.L., Qi, J., Sobrado, P. (2013) Eukaryotic UDP-Galactopyranose Mutases are Bi-functional Enzymes: Insights into a Unique Non-redox Reaction. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 67-73.Raleigh, NC: Lulu
43. Sobrado, P. (2013) Flavin-Dependent N-Hydroxylating monooxygenases in Bacterial and Fungi Siderophore Biosynthesis. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 265-276.Raleigh, NC: Lulu
43. Sobrado, P. (2013) Flavin-Dependent N-Hydroxylating monooxygenases in Bacterial and Fungi Siderophore Biosynthesis. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 265-276.Raleigh, NC: Lulu
42. Romero, E., Avila, D., Sobrado, P. (2013) Effect of pH on the Reductive and Oxidative Half-Reactions of Aspergillus fumigatus Siderophore A. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 289-294.Raleigh, NC: Lulu
romero_.pdf |
41. Badieyan, S., Sobrado, P. (2013) Inhibition of siderophore biosynthesis by targeting A. fumigatus ornithine hydroxylase: A structure-based virtual screening study. In Microbial Pathogens and Strategies to Combat Them: Science, Technology and Education. Mendez-Vilas, A., Ed. Formatex Research Center. Badajoz, Spain.430-438
badieyan_s.pdf |
40. Robinson, R., Sobrado, P. (2013) Flavin-Dependent Monooxygenases in Siderophore Biosynthesis (2012) In Flavin and Flavoprotein Handbook. De Gruytler. (Hille, Miller, Palfey Eds). pp. 29-50.
nmo_review.pdf |
39. Oppenheimer, M., Valenciano, A.L., Qi, J., Sobrado, P. (2013) Eukaryotic UDP-Galactopyranose Mutases are Bi-functional Enzymes: Insights into a Unique Non-redox Reaction. In Flavins and Flavoproteins (Miller, S., Hille, R., Palfey, B., Eds). 67-73.Raleigh, NC: Lulu
38. Robinson, R., Basieyan, S., Sobrado, P. (2013) C4a-hydroperoxyflavin formation in N-hydroxylating flavin monooxygenases is mediates by the 2’-OH of the nicotinamide ribose of NADP+. Biochemistry. 52. 9089-9091. Rapid Report
bi4014903.pdf |
37. Komachi, Y., Hatakeyama, S., Motomatsu, H., Futagami, T., Kizjakina, K., Sobrado, P., Ekino, K., Takegawa, K., Goto, M., Nomura, Y., Oka, T. (2013) gfsA encodes a novel galactofuranosylatransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus . Molecular Microbiology. 90. 1054-73.
mmi12416.pdf |
36. Shirey, C., Badeyan, S., Sobrado, P. (2013) Role of S257 in the sliding mechanism of NADP(H) in the reaction catalyzed by A. fumigatus flavin-dependent ornithine N5- monooxygenase SidA. J. Biol. Chem. 288:32440-8
shireyjbc_2013.pdf |
35. Da Fonseca, I., Kizjakina, K., Sobrado, P. (2013). UDP-galactopyranose mutases from Leishmania species that cause visceral and cutaneous leishmaniasis. Arch. Biochem. Biophys. 538. 103-10.
lugm_final.pdf |
34. Boechi, L., de Oliveira, C.A., da Fonseca, I., Kizjakina, K., Sobrado, P., Tanner, J.J., McCammon J.A, (2013) Substrate-dependent dynamics of UDP-Galactopyranose Mutase: implications for drug design. Protein Science. 11. 1490-501.
tcugmps.pdf |
33. Kizjakina, K., Tanner, J.J., Sobrado, P. (2013). Targeting UDP-galactopyranose Mutases from Eukaryotic Human Pathogen. Current Pharmaceutical Design. 19. 2561-73. Review
ugm_review.pdf |
32. Han, A., Robinson, R., Badieyan, S., Ellerbrock, J. Sobrado, P. (2013) Tryptophan-47 in the active site of Methylophaga sp. Strain SK1 flavin monooxygenase is important for hydride transfer. Arch. Biochem. Biophys. 532. 46-53.
fmow47.pdf |
2012
31. Dhatwalia R, Singh H, Solano LM, Oppenheimer M, Robinson RM, Ellerbrock JF, Sobrado P, Tanner JJ.(2012) Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase. J Am Chem Soc. 134:18132-8.
30. Sobrado, P., Noncanonical reactions of flavoenzymes. (2012) International Journal of Molecular Sciences. 13, 14219-14242. Article
ijms-13-14219.pdf |
29. Franceschini S, Fedkenheuer M, Vogelaar NJ, Robinson HH, Sobrado P, Mattevi A. (2012) Structural Insight into the Mechanism of Oxygen Activation and Substrate Selectivity of Flavin-Dependent N-Hydroxylating Monooxygenases. Biochemistry. 51(36):7043-5. Abstract
bi301072w.pdf |
28. Dhatwalia, R., Singh, H., Oppenheimer, M., Sobrado, P., Tanner, J.J. (2012) Crystal Structures of Trypanosoma cruzi UDP-Galctopyranose Mutase Implicate Flexibility of the Histidine Loop in Enzyme Activation. Biochemistry. 51. 4968-79.
Abstract
Abstract
bi300498c.pdf |
27. Romero, E., Fedkenheuer, M., Chocklett, S.W., Qi, J., Oppenheimer, M., Sobrado, P., (2012) Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase. Biochim. Biophys. Acta. 6. 850-7. Abstract
abbfinal.pdf |
26. Qi, J., Robinson, R., Sobrado, P. (2012) A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases. Analytical Biochemistry. 425. 80-7. Abstract
yabio_10893-final.pdf |
25. Romero, E., Robinson, R., Sobrado, P., (2012) Monitoring the reductive and oxidative half-reactions of a flavin dependent monooxygenase using stopped-flow spectrophotometer. JoVe. 61. Abstract
24. Oppenheimer, M., Valenciano, A.L., Kizjakina, K., Qi, J. Sobrado, P., (2012) Chemical mechanism of UDP-galactopyranose mutase from Trypanosoma cruzi: a potential drug target against Chagas’ disease, PLoS ONE. 7(3) e32918. Abstract
journal.pone.0032918.pdf |
23. Dhatwalia, R., Singh, H., Oppenheimer, M., Karr, D.B., Nix, J.C., Sobrado, P., Tanner, J.J., (2012) Crystal structures and small-angle X-ray scattering analysis of UDP-galactopyranose mutase from the pathogenic fungus Aspergillus fumigatus, J. Biol. Chem. 286. 9041-51. Abstract
j._biol._chem.-2012-dhatwalia-9041-51.pdf |
2011
22. Robinson, R., Sobrado, P., (2011) Substrate binding modulates the activity of Mycobacterium smegmatis G (MbsG), a flavin-dependent monooxygenase involved in the biosynthesis of hydroxamate-containing siderophores. Biochemistry, 50. 8489-96. Abstract
bi200933h.pdf |
21. Qi, J., Oppenheimer, M., Sobrado, P. (2011) Fluorescence Polarization Binding assay for Aspergillus fumigatus virulence factor UDP-galactopyranose mutase. Enzyme Research. vol. 2011, Article ID 513905, 9 pages. Abstract
qi_jun_2011.pdf |
20. Oppenheimer, M., Valenciano, A.L., Sobrado, P. (2011) Biosynthesis of Galactofuranose biosynthesis in kinetoplastids: Novel Therapeutics targets for treating Leishmaniasis and Chagas’ Disease. Enzyme Research. vol. 2011, Article ID 415976, 13 pages. Abstract
415976.pdf |
19. Oppenheimer, M., Valenciano, A.L., Sobrado, P. (2011) Isolation and characterization of functional Leishmania major virulence factor UDP-galactopyranose mutase. Biochem Biophys Res Commun. 407. 552-6. Abstract
lmugmbbrc.pdf |
2007-2010
18. Sobrado., P. (2010) Teaching principles of Enzyme Structure, Evolution, and Catalysis Using Bioinformatics. KMB Journal of Science Education. 1.7-12. Abstract
sobrado18.pdf |
17. Chocklett, W.S, Sobrado, P. (2010) Aspergillus fumigatus SidA is a specific ornithine hydroxylase with bound flavin cofactor. Biochemistry. 49. 6777-83. Abstract
afsidabiochem.pdf |
16. Oppenheimer, M.,Polin, M.B., Lowary, T.L., Helm, F. R., Sobrado, P. (2010) Characterization of UDP-Galactopyranose Mutase From Aspergillus fumigatus. Archives of Biochemistry and Biophysics 502. 31-38. Abstract
afugm1.pdf |
15. Oppenheimer, M., Pierce, S.B., Crowford, A.J., Ray, K., Helm, R., Sobrado, P. (2010) Recombinant expression, purification, and characterization of ThmD, the oxidoreductase component of tetrahydrofuran monooxygenase.Archives of Biochemistry and Biophysics. 496. 123-31. Abstract
abbthmd.pdf |
14. Sobrado, P. (2008) Functional Expression and Purification of UDP-Galactopyranose Mutase from Trypanosoma cruzi. In Flavins and Flavoproteins (Frago, S., Gomez-Moreno, C, Medina, M. eds.) 509-513.
13. LeBlanc-Straceski, J., Sokac, A., Bement, W., Sobrado, P., Lemoine, L., (2009) Developmental Expression of Xenopus Myosin 1d (XlMyo1d) and Identification of a Myo1d tail Homology that Overlaps TH1. Develop. Growth Differ. 51. 443-51.
xl_myo1d_paper_in_dgd.pdf |
12. Sobrado, P., Goren, M.A., James, D., Amundson, C.K., Fox, B.G. (2008) A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr. Purif. 2. 229-41. Abstract
1-s2.0-s1046592807002963-main.pdf |
11. Tsai, C-L., Gokulan, K., Sobrado, P., Sacchettini, J.C. and Fitzpatrick, F.P. (2007) Mechanistic and Structural Studies of H373Q Flavocytochrome b2. Biochemistry.46.7844-7851. Abstract
sobrado_2007.pdf |