A main research focus in our laboratory is the study of the mechanism of action of enzymes essential for viability in several human pathogens. The long-term goal is to design specific inhibitors that will be used as chemotherapeutic drugs. We are also studying enzymes involved in the biosynthesis of compounds with medicinal activities, are involved in antibiotic resistant, or in dehalogenation reactions. These studies integrate techniques from molecular biology, protein expression and purification, steady and pre-steady state kinetics, computational modeling, x-ray crystallography, mass spectrometry, UV/visible, and fluorescence spectroscopies. Five different projects are currently being developed.
Flavin-dependent S-monooxygenases
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